cell lines raw264 7 atcc tib 71 hek293t cells atcc crl 11268 thp1 cells atcc tib 202 hl60 cells atcc ccl  (ATCC)

Journal: Frontiers in Immunology

Article Title: STING activation by teniposide: a potential direct mechanism beyond cGAS stimulation

doi: 10.3389/fimmu.2025.1677836

Figure Lengend Snippet: Teniposide is a potential STING agonist that induces IFNB1 gene expression. (a) Schematic representation of high-throughput virtual screening HTVS of NIH libraries targeting the STING binding pocket. (b) Representation of the 2D structures of Teniposide and cGAMP. (c) Relative IFNB1 gene expression in THP1 cells treated with varying doses of Teniposide or cGAMP. (d) Time-course IFNB1 gene expression in THP1 cells treatment with cGAMP or Teniposide. (e) MX1 and IL6 gene expression in THP1 cells 12h post-Teniposide treatment. (f) Dose response of Teniposide in BMDMs, showing relative IFNB1 expression 24h post-treatment. (g) IFNB1 time-course expression in BMDM upon stimulation with Teniposide (1µM or 3µM). cGAMP induction at 8h was used as a reference control. (h) Relative MX1 and IL6 gene expression in BMDM 48h after Teniposide treatment. Statistical significance is indicated by *p ≤ 0.05, **p ≤ 0.01, ***p ≤0.005, **** p ≤ 0.001.

Article Snippet: Thp1 ATCC ® TIB-202TM cell cultures are human monocyte cells obtained from peripheral blood of patients with acute monocytic leukemia.

Techniques: Gene Expression, High Throughput Screening Assay, Binding Assay, Expressing, Control

Journal: Frontiers in Immunology

Article Title: STING activation by teniposide: a potential direct mechanism beyond cGAS stimulation

doi: 10.3389/fimmu.2025.1677836

Figure Lengend Snippet: Teniposide-induced IFNB1 expression requires STING and may occur independently of cGAS and IFI16. (a) IFNB1 relative gene induction measured by RT-qPCR in WT or STING KO THP1 cells after Teniposide 3µM treatment for 8h. (b) IFNB1 relative expression in WT or GT STING BMDM treated with 3µM Teniposide for 8h. (c) Western blot representing pTBK1, total TBK1, pIRF3, total IRF3 and bacting in THP1cells mock treated, treated for 4h with 3µM of Teniposide or 1µM of diABZI. (d) IFNB1 relative gene expression in WT THP1 cells stimulated for 18h with 10 IU of IFN-β/ml or mock-treated followed by a treatment with DMSO, cGAMP or Teniposide (3µM). (e) Same as in (d) but in WT and cGAS KO THP1 cells. (f) Same as in (d) but in WT and IFI16 THP1 KO cells. (g) Western blot verifying STING, cGAS or IFI16 in WT but not STING, cGAS, and IFI16 KO THP1 cells. Tubulin or β-Actin is shown as a loading control. Statistical significance is indicated by *p ≤ 0.05, **p ≤ 0.01, ***p ≤0.005, **** p ≤ 0.001.

Article Snippet: Thp1 ATCC ® TIB-202TM cell cultures are human monocyte cells obtained from peripheral blood of patients with acute monocytic leukemia.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Gene Expression, Control

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: GATA2 Mediates Macrophage Proliferation During Atherosclerosis

doi: 10.1161/JAHA.125.043646

Figure Lengend Snippet: A , Cytoscape network analysis identified enrichment of a proliferative network in the transcriptome of GATA2+ macrophages compared with monocytes from healthy controls. B , Overexpression of GATA2 in THP1‐derived macrophages increases the expression of MYB and decreases the expression of JUN compared with wild‐type THP1 macrophages. C and D , Quantification of JUN ( C ) and MYB ( D ) promoter activity using firefly luciferase reporters containing the MYB or JUN promoters, in WT vs GATA2‐overexpressing (GATA2) THP1‐derived macrophages. Cells are either untransfected (Mock), transfected with a promoterless vector (Empty) or transfected with a MYB‐ or JUN‐promoter containing vector. E , MYB expression measured by RT‐PCR in WT, GATA2‐overexpressing (GATA2), and GATA2‐shRNA expression (shGATA2) THP1 monocytes and THP1‐derived macrophages. F , Impact of GATA2 on the proliferation rate of GATA2‐overexpressing (GATA2) vs WT THP1 monocytes as they differentiate into macrophages. G , GATA2 expression in THP1‐macrophages following exposure to the indicated concentrations of oxLDL for 48 hours. P values are in comparison to 0 mmol/L. H , Impact of GATA2 and MYB siRNA knockdown, vs a scrambled siRNA control (Scrm), on the proliferation of THP1‐macrophages that are untreated (UT) or treated with oxLDL. I , THP1 proliferation during monocyte–macrophage differentiation and during macrophage‐foam cell differentiation. THP1 cells are either WT or GATA2 overexpressing (GATA2)±treatment with oxLDL during monocyte‐to‐macrophage differentiation (oxLDL), or were first differentiated fully into macrophages before loading with proliferation dye, and proliferation quantified during FC differentiation. Data are presented as the integrated cell proliferation dye intensity of >250 cells. Proliferation is identified as a decrease in the intensity of the cell proliferation dye compared with the fixed control sample (Fixed). * P <0.0001, † P >0.9999 between indicated groups. J , RT‐PCR quantification of GATA2 and MYB expression during the polarization of (HC) vs Pt monocytes into Mφ and FC. K , THP1‐derived macrophage apoptosis under control conditions vs following stimulation with 2.5 mmol/L oxLDL, or 10 μmol/L Tg. Macrophages are either derived from WT THP1 cells (green), GATA2‐overexpressing THP1 cells (magenta), or THP1 cells expressing a GATA2‐depleating shRNA (shGATA2, orange). n=3 ( B , E , I ), 5 ( C , D , H , K ), 6 ( F ), or 4 ( G , J ) biological replicates, Mann–Whitney U test ( B through D , H ) or Kruskal–Wallis test with Dunn correction ( G , I , J ). FC indicates foam cell; HC, healthy control; Mφ, macrophage; oxLDL, oxidized low‐density lipoprotein; Pt, Patient; RT‐PCR, reverse transcription polymerase chain reaction; Tg, thapsigargin; and WT, wild‐type.

Article Snippet: Lympholyte poly and THP1 human monocytic cells (ATCC TIB 202) were purchased from Cedarlane Labs (Burlington, Canada).

Techniques: Over Expression, Derivative Assay, Expressing, Activity Assay, Luciferase, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, shRNA, Comparison, Knockdown, Control, Cell Differentiation, MANN-WHITNEY, Reverse Transcription, Polymerase Chain Reaction